pGEX-T-rnVAPA-1-219
(Plasmid
#13395)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepGEX-4T
- Backbone size w/o insert (bp) 4900
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameVAP-A
-
Alt nameVAPA
-
Alt nameVAP
-
Alt nameVAP-33
-
SpeciesR. norvegicus (rat)
-
Insert Size (bp)662
-
Entrez GeneVapa
-
Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI-modified (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ncbi gi:118142811 / swiss-prot entry Q9Z270
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGEX-T-rnVAPA-1-219 was a gift from Axel Brunger (Addgene plasmid # 13395 ; http://n2t.net/addgene:13395 ; RRID:Addgene_13395) -
For your References section:
Structural basis of FFAT motif-mediated ER targeting. Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT. Structure. 2005 Jul . 13(7):1035-45. 10.1016/j.str.2005.04.010 PubMed 16004875