pIF251
(Plasmid
#133935)
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PurposeKanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2957
- Total vector size (bp) 4547
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Vector typeλ-phage recombineering plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
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Insert Size (bp)1590
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that there are several discrepancies between Addgene's quality control and the depositor's sequence that affects restriction enzyme sites.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIF251 was a gift from IIya Finkelstein (Addgene plasmid # 133935 ; http://n2t.net/addgene:133935 ; RRID:Addgene_133935) -
For your References section:
Efficient modification of lambda-DNA substrates for single-molecule studies. Kim Y, de la Torre A, Leal AA, Finkelstein IJ. Sci Rep. 2017 May 18;7(1):2071. doi: 10.1038/s41598-017-01984-x. 10.1038/s41598-017-01984-x PubMed 28522818
