Skip to main content
Addgene

pIF251
(Plasmid #133935)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 133935 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2957
  • Total vector size (bp) 4547
  • Vector type
    λ-phage recombineering plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
  • Insert Size (bp)
    1590

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are several discrepancies between Addgene's quality control and the depositor's sequence that affects restriction enzyme sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIF251 was a gift from IIya Finkelstein (Addgene plasmid # 133935 ; http://n2t.net/addgene:133935 ; RRID:Addgene_133935)

  • For your References section:

    Efficient modification of lambda-DNA substrates for single-molecule studies. Kim Y, de la Torre A, Leal AA, Finkelstein IJ. Sci Rep. 2017 May 18;7(1):2071. doi: 10.1038/s41598-017-01984-x. 10.1038/s41598-017-01984-x PubMed 28522818
Related items: