pCI-Myc3-HPS4
(Plasmid #133931)

• Purpose: Expresses 3xMyc-HPS4 construct in mammalian cells
• Depositing Lab: Juan Bonifacino
• Publication: Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84. doi: 10.1074/jbc.M301294200. Epub 2003 May 19.

Backbone

• Vector backbone: pCINeo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 7707
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HPS4
• Species: H. sapiens (human)
• Insert Size (bp): 2235
• Mutation: Glutamic acid 229 to Glycine; Valine 552 to Methionine ; Histidine 606 to Tyrosine; Glutamine 625 to Histidine
• GenBank ID: NM_022081
• Entrez Gene: HPS4 (a.k.a. BLOC3S2, LE)
• Promoter: CMV I.E.
• Tag / Fusion Protein:
  • 3xMyc tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: SV40pA reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry