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Addgene

pCI-HA3-HPS1
(Plasmid #133930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133930 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCINeo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Total vector size (bp) 7674
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2202
  • GenBank ID
    NM_000195
  • Entrez Gene
    HPS1 (a.k.a. BLOC3S1, HPS)
  • Promoter CMV I.E.
  • Tag / Fusion Protein
    • 3xHA tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer SV40pA reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-HA3-HPS1 was a gift from Juan Bonifacino (Addgene plasmid # 133930 ; http://n2t.net/addgene:133930 ; RRID:Addgene_133930)

  • For your References section:

    BLOC-3, a protein complex containing the Hermansky-Pudlak syndrome gene products HPS1 and HPS4. Martina JA, Moriyama K, Bonifacino JS. J Biol Chem. 2003 Aug 1;278(31):29376-84. doi: 10.1074/jbc.M301294200. Epub 2003 May 19. 10.1074/jbc.M301294200 PubMed 12756248
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