pCAG-MLC2(CA)-IRES-GFP
(Plasmid
#133928)
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PurposeBicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGEN
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP and MLC2(CA)
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SpeciesH. sapiens (human)
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MutationMLC2 T18D, S19D
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Entrez GeneMYL2 (a.k.a. CMH10, MFM12, MLC-2, MLC-2s/v, MLC-2v, MLC2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-MLC2(CA)-IRES-GFP was a gift from Frank Bradke & Sebastián Dupraz (Addgene plasmid # 133928 ; http://n2t.net/addgene:133928 ; RRID:Addgene_133928) -
For your References section:
RhoA Controls Axon Extension Independent of Specification in the Developing Brain. Dupraz S, Hilton BJ, Husch A, Santos TE, Coles CH, Stern S, Brakebusch C, Bradke F. Curr Biol. 2019 Nov 18;29(22):3874-3886.e9. doi: 10.1016/j.cub.2019.09.040. Epub 2019 Oct 31. 10.1016/j.cub.2019.09.040 PubMed 31679934