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Addgene

pCMVd1-EGFP-hRhoA-wt
(Plasmid #133926)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133926 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    NA
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-hRhoA WT
  • Species
    H. sapiens (human)
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMVd1-EGFP-hRhoA-wt was a gift from Frank Bradke & Sebastián Dupraz (Addgene plasmid # 133926 ; http://n2t.net/addgene:133926 ; RRID:Addgene_133926)

  • For your References section:

    RhoA Controls Axon Extension Independent of Specification in the Developing Brain. Dupraz S, Hilton BJ, Husch A, Santos TE, Coles CH, Stern S, Brakebusch C, Bradke F. Curr Biol. 2019 Nov 18;29(22):3874-3886.e9. doi: 10.1016/j.cub.2019.09.040. Epub 2019 Oct 31. 10.1016/j.cub.2019.09.040 PubMed 31679934
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