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Addgene

pJAB988
(Plasmid #133920)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133920 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1 origin
  • Total vector size (bp) 8143
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miniICE-tet(M)cat(PC194)_PT7-GFP
  • Species
    Synthetic
  • Insert Size (bp)
    6200
  • Promoter T7 promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cgctatgcagatcattaataactatgtaaggttcg
  • 3′ sequencing primer CACTTACTTTAGTTTTATGGAAATGAAAGATCATATCATATATAATCTAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJAB988 was a gift from Christopher Voigt (Addgene plasmid # 133920 ; http://n2t.net/addgene:133920 ; RRID:Addgene_133920)

  • For your References section:

    Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria. Brophy JAN, Triassi AJ, Adams BL, Renberg RL, Stratis-Cullum DN, Grossman AD, Voigt CA. Nat Microbiol. 2018 Sep;3(9):1043-1053. doi: 10.1038/s41564-018-0216-5. Epub 2018 Aug 20. 10.1038/s41564-018-0216-5 PubMed 30127494
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