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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13392 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepZIP-Neo SV(X)1
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Backbone manufacturerCepko et al.
- Backbone size w/o insert (bp) 10200
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Vector typeMammalian Expression, Retroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameC/EBPalpha
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1000
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Entrez GeneCebpa (a.k.a. DBPCEP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPeter Johnson, NCI
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSV(X) C/EBPalpha was a gift from Richard Schwartz (Addgene plasmid # 13392 ; http://n2t.net/addgene:13392 ; RRID:Addgene_13392) -
For your References section:
Redundancy of C/EBP alpha, -beta, and -delta in supporting the lipopolysaccharide-induced transcription of IL-6 and monocyte chemoattractant protein-1. Hu HM, Baer M, Williams SC, Johnson PF, Schwartz RC. J Immunol. 1998 Mar 1. 160(5):2334-42. PubMed 9498774