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Addgene

pET28-His6-GFP-C-LACE
(Plasmid #133913)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133913 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 6051
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    superfolder green fluorescent protein
  • Alt name
    sfGFP
  • Insert Size (bp)
    824
  • Promoter T7
  • Tags / Fusion Proteins
    • LACE tag (GSGPRKVIKMESEE) (C terminal on insert)
    • TEV-cleavable His6-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-His6-GFP-C-LACE was a gift from Jeffrey Bode (Addgene plasmid # 133913 ; http://n2t.net/addgene:133913 ; RRID:Addgene_133913)

  • For your References section:

    Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinant proteins. Hofmann R, Akimoto G, Wucherpfennig TG, Zeymer C, Bode JW. Nat Chem. 2020 Nov;12(11):1008-1015. doi: 10.1038/s41557-020-0528-y. Epub 2020 Sep 14. 10.1038/s41557-020-0528-y PubMed 32929246
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