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pET28-His6-Ubc9-C138A
(Plasmid #133911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133911 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 5773
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SUMO-conjugating enzyme UBC9
  • Alt name
    UBE2I
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    483
  • Mutation
    Cysteine 138 to Alanine
  • Entrez Gene
    UBE2I (a.k.a. C358B7.1, P18, UBC9)
  • Promoter T7
  • Tag / Fusion Protein
    • Thrombin-cleavable His6-tag (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-His6-Ubc9-C138A was a gift from Jeffrey Bode (Addgene plasmid # 133911 ; http://n2t.net/addgene:133911 ; RRID:Addgene_133911)

  • For your References section:

    Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinant proteins. Hofmann R, Akimoto G, Wucherpfennig TG, Zeymer C, Bode JW. Nat Chem. 2020 Nov;12(11):1008-1015. doi: 10.1038/s41557-020-0528-y. Epub 2020 Sep 14. 10.1038/s41557-020-0528-y PubMed 32929246
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