pAD-SPS6-Pman
(Plasmid
#133906)
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PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133906 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAD43-25
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Backbone manufacturerDunn and Handelsman, 1999
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Vector typeBacterial Expression ; E.coli/Bacillus spp. shuttle vector
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Selectable markersAmpicillin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)MC1061
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesfGFP(SPS6)
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SpeciesSynthetic
- Promoter Pman
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer FW
- 3′ sequencing primer RV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAD-SPS6-Pman was a gift from Oscar Kuipers (Addgene plasmid # 133906 ; http://n2t.net/addgene:133906 ; RRID:Addgene_133906) -
For your References section:
Optimized fluorescent proteins for the rhizosphere-associated bacterium Bacillus mycoides with endophytic and biocontrol agent potential. Yi Y, Frenzel E, Spoelder J, Elzenga JTM, van Elsas JD, Kuipers OP. Environ Microbiol Rep. 2018 Feb;10(1):57-74. doi: 10.1111/1758-2229.12607. Epub 2018 Jan 5. 10.1111/1758-2229.12607 PubMed 29195004