Flag-Gsdmd-NT C77A
(Plasmid
#133889)
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PurposeExpresses Gsdmd-NT mutant in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133889 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFlag-CMV-4
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGsdmd-NT
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag-Gsdmd-NT C77A was a gift from Judy Lieberman (Addgene plasmid # 133889 ; http://n2t.net/addgene:133889 ; RRID:Addgene_133889) -
For your References section:
Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores. Liu X, Zhang Z, Ruan J, Pan Y, Magupalli VG, Wu H, Lieberman J. Nature. 2016 Jul 6;535(7610):153-8. doi: 10.1038/nature18629. 10.1038/nature18629 PubMed 27383986