p15A-PB
(Plasmid
#133886)
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Purpose(Empty Backbone) piggyBac ITR's in low copy number p15A backbone plasmid
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133886 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepStart-K
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Backbone manufacturerSen Wu, Capecchi laboratory
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Vector typepiggyBac
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypiggyBac ITR's were synthesized from sequences in the NCBI database.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
AgeI and HindIII sites in the polylinker are present elsewhere in the plasmid as well.
EcoRV site is within the 5' piggyBac ITR sequence.
Please check the purity of the plasmid preparation by agarose gel electrophoresis before using the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p15A-PB was a gift from Mario Capecchi (Addgene plasmid # 133886 ; http://n2t.net/addgene:133886 ; RRID:Addgene_133886)