TB:7xE-Box:MiniP
(Plasmid
#133880)
-
Purpose7 copies of the E-box motif
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 133880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepUPD
-
Backbone manufacturerDiego Orzaez Lab
- Backbone size w/o insert (bp) 3057
- Total vector size (bp) 3394
-
Vector typeMammalian Expression, Luciferase, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert name7 copies of the E-box motif
-
Alt nameTranscription Blocker + 7 copies of the E-box motif + miniP
-
SpeciesH. sapiens (human), Synthetic
-
Insert Size (bp)337
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
- 5′ sequencing primer gtaaaacgacggccagt
- 3′ sequencing primer gtcatagctgtttcctg (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert can be released with BsaI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
TB:7xE-Box:MiniP was a gift from Koen Venken (Addgene plasmid # 133880 ; http://n2t.net/addgene:133880 ; RRID:Addgene_133880) -
For your References section:
Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways. Sarrion-Perdigones A, Gonzalez Y, Venken KJT. Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121. 10.1002/cpmb.121 PubMed 32539183