pcDNA3.1(+)_UGControl
(Plasmid
#133875)
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PurposeExpresses UGControl in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133875 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5373
- Total vector size (bp) 6870
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUtrCH
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Alt nameUtrophin
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SpeciesH. sapiens (human)
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Insert Size (bp)1497
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GenBank IDNM_007124
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Entrez GeneUTRN (a.k.a. DMDL, DRP, DRP1)
- Promoter CMV promoter
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Tag
/ Fusion Protein
- flexible linker - mEGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)_UGControl was a gift from Sumio Terada (Addgene plasmid # 133875 ; http://n2t.net/addgene:133875 ; RRID:Addgene_133875) -
For your References section:
Genetically encoded orientation probes for F-actin for fluorescence polarization microscopy. Nakai N, Sato K, Tani T, Saito K, Sato F, Terada S. Microscopy (Oxf). 2019 Jul 2. pii: 5525243. doi: 10.1093/jmicro/dfz022. 10.1093/jmicro/dfz022 PubMed 31264686