pcDNA3.1(+)_UG3
(Plasmid #133874)

• Purpose: Expresses UG3 in mammalian cells.
• Depositing Lab: Sumio Terada
• Publication: Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 5525243. doi: 10.1093/jmicro/dfz022.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5373
• Total vector size (bp): 6837
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UtrCH
• Alt name: Utrophin
• Species: H. sapiens (human)
• Insert Size (bp): 1464
• GenBank ID: NM_007124
• Entrez Gene: UTRN (a.k.a. DMDL, DRP, DRP1)
• Promoter: CMV promoter
• Tag / Fusion Protein:
  • helical linker - mEGFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH

Resource Information

• Supplemental Documents:
  • pcdna3-1_ug3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry