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Addgene

pJBL7030
(Plasmid #133872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 3096
  • Total vector size (bp) 3967
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtzO-sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    871
  • Promoter modification of pAtzDEF

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A154T mutation in superfolder GFP does not impact plasmid function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBL7030 was a gift from Julius Lucks (Addgene plasmid # 133872 ; http://n2t.net/addgene:133872 ; RRID:Addgene_133872)

  • For your References section:

    Design and Optimization of a Cell-Free Atrazine Biosensor. Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB. ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 10.1021/acssynbio.9b00388. Epub 2020 Mar 3. 10.1021/acssynbio.9b00388 PubMed 32078765
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