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Addgene

pJBL7035
(Plasmid #133870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJL1
  • Backbone size w/o insert (bp) 1763
  • Total vector size (bp) 3209
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grows very slowly--2 days from colony to rich media even when uninduced
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtzB
  • Species
    Pseudomonas ADP-1
  • Insert Size (bp)
    1446
  • Promoter T7

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Was cloned by Twist Biosciences into specified pJL1 vector

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBL7035 was a gift from Julius Lucks (Addgene plasmid # 133870 ; http://n2t.net/addgene:133870 ; RRID:Addgene_133870)

  • For your References section:

    Design and Optimization of a Cell-Free Atrazine Biosensor. Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB. ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 10.1021/acssynbio.9b00388. Epub 2020 Mar 3. 10.1021/acssynbio.9b00388 PubMed 32078765
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