pJFRC-nSyb-IVS-PhiC31
(Plasmid
#133868)
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PurposeReplace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJFRC7
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Backbone manufacturerJanelia
- Total vector size (bp) 11147
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePhiC31 Recombinase
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Insert Size (bp)1842
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Entrez Geneint (a.k.a. phiC31p51)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJFRC-nSyb-IVS-PhiC31 was a gift from Tom Clandinin (Addgene plasmid # 133868 ; http://n2t.net/addgene:133868 ; RRID:Addgene_133868) -
For your References section:
SPARC enables genetic manipulation of precise proportions of cells. Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin TR. Nat Neurosci. 2020 Sep;23(9):1168-1175. doi: 10.1038/s41593-020-0668-9. Epub 2020 Jul 20. 10.1038/s41593-020-0668-9 PubMed 32690967