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Addgene

pDsRed1-N1 RanGAP
(Plasmid #13386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDsRed1-N1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RanGAP
  • Alt name
    RanGAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Entrez Gene
    RANGAP1 (a.k.a. Fug1, RANGAP, SD)
  • Tag / Fusion Protein
    • DsRed1 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDsRed1-N1 RanGAP was a gift from Mary Dasso (Addgene plasmid # 13386 ; http://n2t.net/addgene:13386 ; RRID:Addgene_13386)

  • For your References section:

    SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. Joseph J, Tan SH, Karpova TS, McNally JG, Dasso M. J Cell Biol. 2002 Feb 18. 156(4):595-602. 10.1083/jcb.200110109 PubMed 11854305
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