pmApple-GNG2
(Plasmid
#133857)
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PurposeMammalian expression of GNG2 N-terminally tagged with mApple
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneC1
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Backbone manufacturerClontech
- Total vector size (bp) 5250
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGNG2
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Alt namegamma2
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Alt nameGgamma2
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Alt nameGγ2
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SpeciesH. sapiens (human)
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Entrez GeneGNG2 (a.k.a. HG3F1)
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Tag
/ Fusion Protein
- mApple (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGE Healthcare Life Sciences
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmApple-GNG2 was a gift from Dave Piston (Addgene plasmid # 133857 ; http://n2t.net/addgene:133857 ; RRID:Addgene_133857) -
For your References section:
Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins. Foust DJ, Godin AG, Ustione A, Wiseman PW, Piston DW. Biophys J. 2019 Nov 5;117(9):1764-1777. doi: 10.1016/j.bpj.2019.09.028. Epub 2019 Sep 28. 10.1016/j.bpj.2019.09.028 PubMed 31606123