pEGFP-GNB1
(Plasmid
#133856)
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PurposeMammalian expression of GNB1 N-terminally tagged with EGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133856 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneC1
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Backbone manufacturerClontech
- Total vector size (bp) 5784
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGNB1
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Alt nameGbeta1
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Alt nameGβ1
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SpeciesH. sapiens (human)
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Entrez GeneGNB1 (a.k.a. HG2A, MDS, MRD42)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySteven Vogel via Addgene #27810
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-GNB1 was a gift from Dave Piston (Addgene plasmid # 133856 ; http://n2t.net/addgene:133856 ; RRID:Addgene_133856) -
For your References section:
Two-Color Spatial Cumulant Analysis Detects Heteromeric Interactions between Membrane Proteins. Foust DJ, Godin AG, Ustione A, Wiseman PW, Piston DW. Biophys J. 2019 Nov 5;117(9):1764-1777. doi: 10.1016/j.bpj.2019.09.028. Epub 2019 Sep 28. 10.1016/j.bpj.2019.09.028 PubMed 31606123