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Addgene

pMG36E
(Plasmid #133853)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133853 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWV01
  • Backbone size (bp) 1800
  • Modifications to backbone
    EmR; mcs; P32
  • Vector type
    Bacterial Expression
  • Promoter P32

Growth in Bacteria

  • Bacterial Resistance(s)
    Erythromycin, 200 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    E.coli MC100
  • Growth instructions
    This plasmid can be propagated in a broad host range, including Lactococcus lactis, Escherichia coli, Bacillus subtilis and several Lactobacillus strains.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMG36E was a gift from Jan Kok & Gerard Venema (Addgene plasmid # 133853 ; http://n2t.net/addgene:133853 ; RRID:Addgene_133853)

  • For your References section:

    Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis. van de Guchte M, van der Vossen JM, Kok J, Venema G. Appl Environ Microbiol. 1989 Jan;55(1):224-8. PubMed 2495760
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