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pAD-KPS12-Pman
(Plasmid #133832)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133832 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAD43-25
  • Backbone manufacturer
    Dunn and Handelsman, 1999
  • Vector type
    Bacterial Expression ; E.coli/Bacillus spp. shuttle vector
  • Selectable markers
    Ampicillin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MC1061
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mKate(KPS12)
  • Alt name
    mutated variant of mKate2 ((Piatkevich et al., 2010)
  • Species
    Synthetic
  • Insert Size (bp)
    693
  • Promoter Pman

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer FW
  • 3′ sequencing primer RV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAD-KPS12-Pman was a gift from Oscar Kuipers (Addgene plasmid # 133832 ; http://n2t.net/addgene:133832 ; RRID:Addgene_133832)

  • For your References section:

    Optimized fluorescent proteins for the rhizosphere-associated bacterium Bacillus mycoides with endophytic and biocontrol agent potential. Yi Y, Frenzel E, Spoelder J, Elzenga JTM, van Elsas JD, Kuipers OP. Environ Microbiol Rep. 2018 Feb;10(1):57-74. doi: 10.1111/1758-2229.12607. Epub 2018 Jan 5. 10.1111/1758-2229.12607 PubMed 29195004
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