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pFSW Syt1-C2A M173,F234A IRES GFP
(Plasmid #133825)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133825 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Syt1
  • Alt name
    Synaptotagmin 1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    789
  • Mutation
    M173, F234A
  • Entrez Gene
    Syt1 (a.k.a. P65)
  • Promoter hSyn (human synapsin I promoter)
  • Tag / Fusion Protein
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: NheI (destroyed during cloning), 3' Cloning Site: EcoRI (destroyed during cloning)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFSW Syt1-C2A M173,F234A IRES GFP was a gift from Edwin Chapman (Addgene plasmid # 133825 ; http://n2t.net/addgene:133825 ; RRID:Addgene_133825)

  • For your References section:

    Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin. Courtney NA, Bao H, Briguglio JS, Chapman ER. Nat Commun. 2019 Sep 9;10(1):4076. doi: 10.1038/s41467-019-12015-w. 10.1038/s41467-019-12015-w PubMed 31501440
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