pFSW GFP IRES GFP
(Plasmid
#133815)
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Purpose(Empty Backbone) Encodes EGFP that can be used as a control for viral expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133815 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
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Vector typeLentiviral
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Tag
/ Fusion Protein
- IRES-GFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- IRES-GFP (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: EcoRI (not destroyed)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFSW GFP IRES GFP was a gift from Edwin Chapman (Addgene plasmid # 133815 ; http://n2t.net/addgene:133815 ; RRID:Addgene_133815) -
For your References section:
Synaptotagmin 1 clamps synaptic vesicle fusion in mammalian neurons independent of complexin. Courtney NA, Bao H, Briguglio JS, Chapman ER. Nat Commun. 2019 Sep 9;10(1):4076. doi: 10.1038/s41467-019-12015-w. 10.1038/s41467-019-12015-w PubMed 31501440