pCP-tRNA
(Plasmid
#133812)
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PurposeCRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Vector typeCRISPR
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Selectable markersSAT1 (resistance to Nourseothricin)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
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gRNA/shRNA sequenceUnknown
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid contains the following relevant inserts: 1. C. parapsilosis Autonomously Replicating Sequence 7 for episomal propagation in C. parapsilosis; 2. CAS9 sequence expressed from a C. parapsilosis promoter; 3. Cassette for the expression of the sgRNA from a C. parapsilosis RNA pol II promoter. The mature sgRNA is released following RNase Z processing of the upstream tRNA and self-cleavage of the dowstream HDV Ribozyme. The guide targeting the gene of interest can be cloned into the SapI/BspQI digested plasmid. The regulatory elements (species of origin of promoters/terminators) are described in the map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCP-tRNA was a gift from Geraldine Butler (Addgene plasmid # 133812 ; http://n2t.net/addgene:133812 ; RRID:Addgene_133812) -
For your References section:
Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species. Lombardi L, Oliveira-Pacheco J, Butler G. mSphere. 2019 Mar 13;4(2). pii: 4/2/e00125-19. doi: 10.1128/mSphere.00125-19. 10.1128/mSphere.00125-19 PubMed 30867327