pET30 SUMO-1 G97
(Plasmid #13381)

• Depositing Lab: Mary Dasso
• Publication: Saitoh et al Curr Biol. 1998 Jan 15. 8(2):121-4.

Backbone

• Vector backbone: pET30
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO-1, G97
• Alt name: SUMO-1
• Species: H. sapiens (human)
• Insert Size (bp): 300
• Mutation: G97 mutant: lacks 4 amino acids from the C-terminus
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Tag / Fusion Protein:
  • His

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry