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Purposerepressible expression of CD19 and mCherry in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133803 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHR
- Backbone size w/o insert (bp) 10005
- Total vector size (bp) 819
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameputative ECD of H. sapiens CD19
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SpeciesH. sapiens (human)
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Entrez GeneCD19 (a.k.a. B4, CVID3)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR_TetO_CD19-P2A-mCherry was a gift from Wendell Lim (Addgene plasmid # 133803 ; http://n2t.net/addgene:133803 ; RRID:Addgene_133803) -
For your References section:
Programming self-organizing multicellular structures with synthetic cell-cell signaling. Toda S, Blauch LR, Tang SKY, Morsut L, Lim WA. Science. 2018 Jul 13;361(6398):156-162. doi: 10.1126/science.aat0271. Epub 2018 May 31. 10.1126/science.aat0271 PubMed 29853554