pEYFP SUMO-1
(Plasmid #13380)

• Depositing Lab: Mary Dasso
• Publication: Ayaydin et al Mol Biol Cell. 2004 Dec . 15(12):5208-18.

Backbone

• Vector backbone: pEYFP-C1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO-1
• Species: H. sapiens (human)
• Insert Size (bp): 320
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Tag / Fusion Protein:
  • EYFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: SalI (destroyed during cloning)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry