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pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)
(Plasmid #133798)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133798 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    JDS246 (Plasmid #43861)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human codon optimized AcrIIA1(T114A/F115A)
  • Alt name
    KAC688
  • Species
    Synthetic
  • Mutation
    T114A/F115A
  • Promoter CMV and T7
  • Tag / Fusion Protein
    • NLS(SV40) (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer oBK6928-CCAAGTCTCCACCCCATTGACG
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688) was a gift from Joseph Bondy-Denomy & Benjamin Kleinstiver (Addgene plasmid # 133798 ; http://n2t.net/addgene:133798 ; RRID:Addgene_133798)

  • For your References section:

    Listeria Phages Induce Cas9 Degradation to Protect Lysogenic Genomes. Osuna BA, Karambelkar S, Mahendra C, Christie KA, Garcia B, Davidson AR, Kleinstiver BP, Kilcher S, Bondy-Denomy J. Cell Host Microbe. 2020 Apr 18. pii: S1931-3128(20)30190-6. doi: 10.1016/j.chom.2020.04.001. 10.1016/j.chom.2020.04.001 PubMed 32325050
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