pGEX-4T-2 RanGAP
(Plasmid #13379)

• Depositing Lab: Mary Dasso
• Publication: Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602.

Backbone

• Vector backbone: pGEX-4T-2
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RanGAP
• Alt name: RanGAP1
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Entrez Gene: RANGAP1 (a.k.a. Fug1, RANGAP, SD)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: pGEX 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry