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Addgene

pAAV-EF1a-DIO-tdTomato-WPRE
(Plasmid #133786)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133786 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV-EF1a-double floxed-TdTomato-WPRE
  • Backbone manufacturer
    karl deisseroth
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pAAV-EF1a-DIO-tdTomato-WPRE
  • Insert Size (bp)
    1427

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a T230I mutation in tdTomato. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-DIO-tdTomato-WPRE was a gift from Michael Stryker (Addgene plasmid # 133786 ; http://n2t.net/addgene:133786 ; RRID:Addgene_133786)
  • For your References section:

    Experience-dependent structural plasticity at pre- and postsynaptic sites of layer 2/3 cells in developing visual cortex. Sun YJ, Espinosa JS, Hoseini MS, Stryker MP. Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21812-21820. doi: 10.1073/pnas.1914661116. Epub 2019 Oct 7. 10.1073/pnas.1914661116 PubMed 31591211