pAAV-EF1a-DIO-PSD95-EGFP-WPRE
(Plasmid #133785)

• Purpose: Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell
• Depositing Lab: Michael Stryker
• Publication: Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21812-21820. doi: 10.1073/pnas.1914661116. Epub 2019 Oct 7.

Backbone

• Vector backbone: pAAV-EF1a-double floxed-TdTomato-WPRE
• Backbone manufacturer: karl deisseroth
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PSD95-EGFP
• Species: R. norvegicus (rat)
• Insert Size (bp): 2241
• GenBank ID: NM_019621.1
• Entrez Gene: Dlg4 (a.k.a. Dlgh4, PSD95, Sap90)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Resource Information

• Supplemental Documents:
  • D15 AAV EF1A dble flox PSD95-EGFP WPRE.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains FS793-794LT, A892V, and H932T mutations in the insert sequence, which all fall within EGFP. These mutations are not known to affect plasmid function.