pMK33/intein-Cas9_S219-3XFLAG
(Plasmid
#133782)
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PurposeExpresses human codon-optimized intein-Cas9
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMK33
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuman codon-optimized intein-Cas9
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byHuman codon-optimized intein-Cas9_S219-3XFLAG (Davis et al., 2015) was a kind gift of D. Lui.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: EvNO00483430.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMK33/intein-Cas9_S219-3XFLAG was a gift from Norbert Perrimon (Addgene plasmid # 133782 ; http://n2t.net/addgene:133782 ; RRID:Addgene_133782) -
For your References section:
Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells. Viswanatha R, Li Z, Hu Y, Perrimon N. Elife. 2018 Jul 27;7. pii: 36333. doi: 10.7554/eLife.36333. 10.7554/eLife.36333 PubMed 30051818
