pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat
(Plasmid #133779)

• Purpose: Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
• Depositing Lab: Maria Castro
• Publication: pKT-mIDH1(R132H)-Myc-FLAG-IRES-Katushka (unpublished)

Backbone

• Vector backbone: pKT
• Backbone size w/o insert (bp): 4828
• Total vector size (bp): 6307
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Isocitrate dehydrogenase 1
• Species: H. sapiens (human)
• Insert Size (bp): 1479
• Mutation: Arginine is mutated to histidine at amino acid position 132 on IDH1
• GenBank ID:
• Entrez Gene: IDH1 (a.k.a. HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD)
• Tag / Fusion Protein:
  • Myc and FLAG (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pkt-midh1-myc-flag-kat.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry