pcDNA3-6XHis-human SUMO 2
(Plasmid #133771)

• Purpose: express 6Xhis- SUMO 2 protein
• Depositing Lab: Martine Roussel
• Publication: Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 102(21):7689-94.

Backbone

• Vector backbone: pcDNA 3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO 2
• Alt name: BC107853
• Species: H. sapiens (human)
• Insert Size (bp): 312
• Entrez Gene: SUMO2 (a.k.a. HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6XHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV Prom Forw
• 3′ sequencing primer: BGH Rev

Resource Information

• Supplemental Documents:
  • pcDNA3-6xHis-Human SUMO 2 BC107853.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry