pcDNA3-6XHis-human SUMO 1
(Plasmid #133770)

• Purpose: express 6Xhis- SUMO 1 protein
• Depositing Lab: Martine Roussel
• Publication: Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 102(21):7689-94.

Backbone

• Vector backbone: pcDNA 3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO 1
• Alt name: BC053528
• Species: H. sapiens (human)
• Insert Size (bp): 325
• Entrez Gene: SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6XHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV Prom Forw
• 3′ sequencing primer: BGH Rev

Resource Information

• Supplemental Documents:
  • pcDNA3-6xHis-human SUMO 1 BC053528.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry