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Addgene

6599-SUMO1GG
(Plasmid #13377)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4064
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUMO1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • Mutation
    mature form ending with GG
  • Entrez Gene
    SUMO1 (a.k.a. DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6599-SUMO1GG was a gift from Anne Dejean (Addgene plasmid # 13377 ; http://n2t.net/addgene:13377 ; RRID:Addgene_13377)

  • For your References section:

    Common properties of nuclear body protein SP100 and TIF1alpha chromatin factor: role of SUMO modification. Seeler JS, Marchio A, Losson R, Desterro JM, Hay RT, Chambon P, Dejean A. Mol Cell Biol. 2001 May . 21(10):3314-24. 10.1128/MCB.21.10.3314-3324.2001 PubMed 11313457
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