pCB-SC101-Spe
(Plasmid
#133765)
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Purpose(Empty Backbone) shuttle vector backbone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133765 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSC101
- Backbone size (bp) 1900
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Modifications to backboneadd pmlI cutting site for inserting genes
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Vector typeSynthetic Biology ; shuttle vector for E.coli and Synechocystis sp. PCC 6803
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Selectable markersSpe
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberLow Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCB-SC101-Spe was a gift from Himadri Pakrasi (Addgene plasmid # 133765 ; http://n2t.net/addgene:133765 ; RRID:Addgene_133765) -
For your References section:
Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803. Liu D, Pakrasi HB. Microb Cell Fact. 2018 Mar 26;17(1):48. doi: 10.1186/s12934-018-0897-8. 10.1186/s12934-018-0897-8 [pii] PubMed 29580240