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Addgene

pCB-SC101-Spe
(Plasmid #133765)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133765 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSC101
  • Backbone size (bp) 1900
  • Modifications to backbone
    add pmlI cutting site for inserting genes
  • Vector type
    Synthetic Biology ; shuttle vector for E.coli and Synechocystis sp. PCC 6803
  • Selectable markers
    Spe

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB-SC101-Spe was a gift from Himadri Pakrasi (Addgene plasmid # 133765 ; http://n2t.net/addgene:133765 ; RRID:Addgene_133765)
  • For your References section:

    Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803. Liu D, Pakrasi HB. Microb Cell Fact. 2018 Mar 26;17(1):48. doi: 10.1186/s12934-018-0897-8. 10.1186/s12934-018-0897-8 [pii] PubMed 29580240