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Addgene

PWPXLD-GFP-Numb-S276D
(Plasmid #133752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PWPXLD
  • Backbone size w/o insert (bp) 10455
  • Total vector size (bp) 12417
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NUMB endocytic adaptor protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1962
  • Mutation
    serine 276 converted to aspartic acid
  • GenBank ID
    NM_001136075.2
  • Entrez Gene
    Numb (a.k.a. Nb)
  • Promoter EF1-alpha
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PME1 (destroyed during cloning)
  • 3′ cloning site SMA1 (destroyed during cloning)
  • 5′ sequencing primer CAAGCCTCAGACAGTGGTTC
  • 3′ sequencing primer GCGTAAAAGGAGCAACATAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PWPXLD-GFP-Numb-S276D was a gift from Chay Kuo (Addgene plasmid # 133752 ; http://n2t.net/addgene:133752 ; RRID:Addgene_133752)

  • For your References section:

    EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation. Abdi K, Neves G, Pyun J, Kiziltug E, Ahrens A, Kuo CT. Cell Rep. 2019 Aug 20;28(8):2012-2022.e4. doi: 10.1016/j.celrep.2019.07.056. 10.1016/j.celrep.2019.07.056 PubMed 31433979
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