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MSCV-human CSFI-R-L301S- Y969F-IRES-GFP
(Plasmid #133745)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV LTR
  • Backbone size w/o insert (bp) 6415
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CSF1-R
  • Alt name
    c-fms
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Mutation
    L301S- Y969F
  • Entrez Gene
    CSF1R (a.k.a. BANDDOS, C-FMS, CD115, CSF-1R, CSFR, FIM2, FMS, GPSC, HDLS, HDLS1, M-CSF-R)
  • Promoter MSCV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-human CSFI-R-L301S- Y969F-IRES-GFP was a gift from Martine Roussel (Addgene plasmid # 133745 ; http://n2t.net/addgene:133745 ; RRID:Addgene_133745)

  • For your References section:

    Transforming potential of the c-fms proto-oncogene (CSF-1 receptor). Roussel MF, Dull TJ, Rettenmier CW, Ralph P, Ullrich A, Sherr CJ. Nature. 1987 Feb 5-11;325(6104):549-52. 10.1038/325549a0 PubMed 3027579
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