pSG5 E107K T Ag
(Plasmid
#133710)
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PurposeLarge T antigen mutant form in pSG5 vector for mammalian expression.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG5
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSV40gp6
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SpeciesSV40
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MutationE107K
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Entrez GeneSV40gp6 (a.k.a. SV40gp6)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: SvCD00017290
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5 E107K T Ag was a gift from James A. DeCaprio (Addgene plasmid # 133710 ; http://n2t.net/addgene:133710 ; RRID:Addgene_133710) -
For your References section:
Role of pRb-related proteins in simian virus 40 large-T-antigen-mediated transformation. Zalvide J, DeCaprio JA. Mol Cell Biol. 1995 Oct . 15(10):5800-10. PubMed 7565733