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pGL2-basic mt E2F-luciferase
(Plasmid #133708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133708 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5597
  • Vector type
    Mammalian Expression ; trxn factor bind site

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E2F1
  • Species
    H. sapiens (human)
  • Mutation
    the three binding site copies are mutated form
  • Entrez Gene
    E2F1 (a.k.a. E2F-1, RBAP1, RBBP3, RBP3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: HsBS00017288. TATA oligo is GATCTACTTGGGCATAAAGGCAGAGCAGGGCAGCTGCTGCTTACACTTA; 3xE2F-MUTANT oligo is CTGCAATTGCTCGACCAACTTGTGCAATTGCTCGACCAACTTGTGCAATTGCTCGACCAACTTGC.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-basic mt E2F-luciferase was a gift from James A. DeCaprio (Addgene plasmid # 133708 ; http://n2t.net/addgene:133708 ; RRID:Addgene_133708)

  • For your References section:

    The J domain of simian virus 40 large T antigen is required to functionally inactivate RB family proteins. Zalvide J, Stubdal H, DeCaprio JA. Mol Cell Biol 1998 Mar;18(3):1408-15. PubMed 9488456
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