pCMV HA-DP-2
(Plasmid
#133706)
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PurposeExpression of HA-tagged TFDP2.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV4
- Backbone size w/o insert (bp) 4900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTFDP2
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SpeciesH. sapiens (human)
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Entrez GeneTFDP2 (a.k.a. DP2)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: HsCD00017286
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV HA-DP-2 was a gift from James A. DeCaprio (Addgene plasmid # 133706 ; http://n2t.net/addgene:133706 ; RRID:Addgene_133706) -
For your References section:
The subcellular localization of E2F-4 is cell-cycle dependent. Lindeman GJ, Gaubatz S, Livingston DM, Ginsberg D. Proc Natl Acad Sci U S A. 1997 May 13. 94(10):5095-100. 10.1073/pnas.94.10.5095 PubMed 9144196