pCMV HA-DP-1
(Plasmid
#133705)
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PurposeExpression of HA-tagged TFDP1.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 133705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV4
- Backbone size w/o insert (bp) 4900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTFDP1
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SpeciesH. sapiens (human)
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GenBank IDNP_001278694.1
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Entrez GeneTFDP1 (a.k.a. DILC, DP1, DRTF1, Dp-1)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: HsCD00017285
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV HA-DP-1 was a gift from James A. DeCaprio (Addgene plasmid # 133705 ; http://n2t.net/addgene:133705 ; RRID:Addgene_133705) -
For your References section:
Binding to DNA and the retinoblastoma gene product promoted by complex formation of different E2F family members. Krek W, Livingston DM, Shirodkar S. Science. 1993 Dec 3. 262(5139):1557-60. 10.1126/science.8248803 PubMed 8248803