ZJOM143
(Plasmid
#133667)
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PurposeIntegration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneClhn-mTagBFP2-Trp
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep1k-VPH1-mTagBFP2
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3500
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Entrez GeneVPH1 (a.k.a. YOR270C)
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Tag
/ Fusion Protein
- mTagBFP2 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZJOM143 was a gift from Zhiping Xie (Addgene plasmid # 133667 ; http://n2t.net/addgene:133667 ; RRID:Addgene_133667) -
For your References section:
A Validated Set of Fluorescent-Protein-Based Markers for Major Organelles in Yeast (Saccharomyces cerevisiae). Zhu J, Zhang ZT, Tang SW, Zhao BS, Li H, Song JZ, Li D, Xie Z. MBio. 2019 Sep 3;10(5). pii: mBio.01691-19. doi: 10.1128/mBio.01691-19. 10.1128/mBio.01691-19 PubMed 31481383
