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Addgene

ZJOM20
(Plasmid #133644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 133644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    KT209-2GFP
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p1k-PEX1-2GFP
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    4100
  • Entrez Gene
    PEX1 (a.k.a. YKL197C, PAS1)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZJOM20 was a gift from Zhiping Xie (Addgene plasmid # 133644 ; http://n2t.net/addgene:133644 ; RRID:Addgene_133644)

  • For your References section:

    A Validated Set of Fluorescent-Protein-Based Markers for Major Organelles in Yeast (Saccharomyces cerevisiae). Zhu J, Zhang ZT, Tang SW, Zhao BS, Li H, Song JZ, Li D, Xie Z. MBio. 2019 Sep 3;10(5). pii: mBio.01691-19. doi: 10.1128/mBio.01691-19. 10.1128/mBio.01691-19 PubMed 31481383
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