pET28a-MinC
(Plasmid #133628)

• Purpose: Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.
• Depositing Lab: Petra Schwille
• Publication: Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-83. doi: 10.1038/nsmb.2037. Epub 2011 Apr 24.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5245
• Total vector size (bp): 6034
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: His-MinC
• Species: E. coli
• Insert Size (bp): 789
• Entrez Gene: minC (a.k.a. b1176, ECK1164, minB)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pET28a-MinC.gb
• A portion of this plasmid was derived from a plasmid made by: MinC was obtained from plasmid pZH101 originally from D. RayChaudhuri.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry