pET28a-2xMreBN-mCherry-His
(Plasmid
#133626)
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PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133626 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
- Backbone size w/o insert (bp) 5206
- Total vector size (bp) 6052
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name2xMreB-mCherry-His
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Insert Size (bp)846
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Tag
/ Fusion Protein
- His (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-2xMreBN-mCherry-His was a gift from Petra Schwille (Addgene plasmid # 133626 ; http://n2t.net/addgene:133626 ; RRID:Addgene_133626) -
For your References section:
The MinDE system is a generic spatial cue for membrane protein distribution in vitro. Ramm B, Glock P, Mucksch J, Blumhardt P, Garcia-Soriano DA, Heymann M, Schwille P. Nat Commun. 2018 Sep 26;9(1):3942. doi: 10.1038/s41467-018-06310-1. 10.1038/s41467-018-06310-1 PubMed 30258191
Map uploaded by the depositor.
